Table 1 Comparison of the quality of methylome libraries generated by snmC-seq, snmC-seq2, and MethylC-seq

From: Robust single-cell DNA methylome profiling with snmC-seq2

Tissue

FC

MOp

Exc

DNA input

1 cell

1 cell

>50 ng

Method

snmC-seq

snmC-seq2

MethylC-seq

Adaptor dimer and short insert

29.2 ± 16.5%

6.1 ± 5.2%

0.45 ± 0.01%

Mapping

52 ± 4%a

64.7 ± 2.6a

76.4 ± 0.35b

Library complexity

22.2 ± 5.7%c

30.8 ± 7.5%c

N/A

  1. FC mouse frontal cortex; MOp mouse primary motor cortex; Exc Camk2a+-expressing mouse cortical excitatory neurons
  2. aMapping rate computed from both forward and reverse reads
  3. bOnly forward reads were generated
  4. cCoverage of mouse genome
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