Fig. 2
Overview of the SPARTA process flow and trapping control validation. a Process flow starting with the set-up of the acquisition parameters (I) and thresholding based on the signal obtained from an initial particle trap to set the peak position and height used to determine a successful particle trap (II). Next, the acquisition loop is initiated (III, IV) where quick scans are iterated until a trap is recognized (V) after which a high SNR acquisition is taken and saved (VI). The laser is disabled (III) to allow the particle to diffuse away and the process is repeated. After na acquisitions the data can be processed (VII) for further analysis. b Iteration number distribution when rapidly blinking the laser after each trap. c Iteration number distribution when turning the laser off for 0.5 s. d Box and whisker plot showing the signal intensity distribution for a characteristic peak versus the iteration number of the trap, no significant influence was found between any of the iteration numbers >1. One way ANOVA, with Holm-corrected multiple comparisons test, n = 355 combined traps. Indicated are the median (horizontal line inside the box), mean (□), minimum and maximum values as the ends of the whiskers and outliers (♦)
