Fig. 4
From: Clonal dynamics in osteosarcoma defined by RGB marking
Orthotopically implanted RAINBONE cells generate polyclonal tumours. a Tumour developing in the medullar space (orange arrow). Blue section indicates the visual plane for haematoxylin/eosin staining (HE) and confocal images. XR X-ray imaging. b Representative image of RAINBONE cells located in the endosteal space, at perivascular locations, and in compact bone (white arrows). c Detail view of picture B (Box 1) showing osteoblastic rimming (*) and malignant trabecular bone (+). d Osteolytic tumour overgrowing as a soft tissue mass at 50 days presented strong periosteal reaction and a Codman triangle (orange arrows). e Representative image of a large bi-clonal area growing on the outer surface of bone; two different dominant clones are developing in opposition (white line indicates the border limits). f Detailed view of cartilage plate infiltration and destruction. g Picture detail (Box 2) of the clonal growth in the surrounding of musculoskeletal tissue, with low-frequency clones (white arrow) among larger monoclonal areas. White and black bars = 200 µm; blue bars = 50 µm; n = 5
