Fig. 6
From: Clonal dynamics in osteosarcoma defined by RGB marking
Isolated clones from RAINBONE tumours regenerate polyclonal tumours. a Experimental design of RAINBONE subclone recolouring and their in vivo tumourigenic potential (secondary n = 9, tertiary n = 9). The 3D flow cytometry analysis (b) and confocal image (c) of representative RAINBONE FACS sorted clones (n = 3). d Representative 3D flow cytometry analysis of decoloured clones after Ad-Cre transduction. Representative 3D flow cytometry analysis (e) and confocal microscopy image (f) of RGB-marked (recoloured) FACS sorted clones (RAINBONE-2). Representative 3D flow cytometry analysis (g) and confocal microscopy image (h) of RAINBONE-2 tumours (15 days of secondary transplantation) showing a polyclonal population. Representative 3D flow cytometry analysis (i) and confocal microscopy image (j) of RAINBONE-2 tumours (15 days after tertiary transplantation) again showing a polyclonal contribution. Blue bars = 50 µm
