Fig. 4

GTF2H1 expression rescues TFIIH in BRM/BRG1 depleted cells. Representative IF of XPD recruitment (red channel) to LUD marked by XPC (cyan channel). U2OS cells were fixed 30 min after local UV-C irradiation (60 J/m²) through a microporous membrane (8 µm). a U2OS cells were treated with control (CTRL), BRM, or GTF2H1 siRNAs and transiently transfected with TFIIH subunits XPB or GTF2H1 fused to GFP (green channel). Scale bar: 10 µm. b Quantification of XPD recruitment to LUD. Relative accumulation at LUD (over nuclear background) in each condition was normalized to control (siCTRL without transient transfection of TFIIH subunits, indicated by “empty“ symbol), in which nuclear background was set at 0 and maximal signal at LUD set to 1.0 (>50 cells per sample, mean & S.E.M. from four independent experiments). ***P < 0.001, relative to siCTRL without transient transfection of TFIIH subunits. c U2OS cells were treated with siRNA against BRG1 and transiently transfected with TFIIH subunits XPB or GTF2H1 fused to GFP (green channel). Scale bar: 10 µm. Arrows highlight LUD in a mixed population of non-transfected and transfected cells with GFP-GTF2H1 or XPB-GFP (green cells in the right panel). n.s. non-significant