Fig. 2

MARF1^null female flies are sterile and have abnormal stage 14 oocytes. a Male fertility assay. Numbers of progeny flies from crosses between test males and OregonR wild-type females are shown. Mean ± SD (n = 10). b, c Female fertility assays. b The numbers of eggs laid by test females crossed with OregonR wild-type males and c the hatching rates of the eggs. Mean ± SD (n = 3). P-value < 0.05 (Student’s t-test) are indicated by *. d Stereomicroscope images of dissected whole ovaries. Scale bar is 500 μm. e Stereomicroscope images of dissected stage 14 oocytes. Abnormal yolk distribution in MARF1^null stage 14 oocyte is indicated by red arrows. The percentages of normal oocytes are shown. The numbers of normal oocytes/the numbers of observed oocytes are shown in parenthesis. Germline expression of the MARF1 transgene (MAT15Tub-Gal4 → UASP-3xHA-MARF1) in the MARF1^null background rescued hatching rate and yolk distribution in stage 14 oocytes. Scale bar is 100 μm