Fig. 3
From: Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells
Acute deletion of Pkm2 in mouse endothelial cells leads to angiogenic sprouting defects. a Representative confocal projections of PECAM, ERG and EdU immunostained retinas from control and Pkm2iEC-KO mice (A, Artery; V, Vein). b Radial outgrowth in control and Pkm2iEC-KO mice (n = 6). c Representative confocal projections of angiogenic front vessels in PECAM, ERG and EdU-stained retinas from control and Pkm2iEC-KO mice. d ERG+ ECs, EdU+ ECs, EC area per field and branch point density at the angiogenic front of control and Pkm2iEC-KO mice (n = 6). All analyses in the mouse retina were performed on postnatal day 7. Data in b and d represent means ± s.e.m. (**P < 0.01, *P < 0.05 by two-tailed Student's t test). Scale bars in a and c = 200 μm
