Fig. 1 | Nature Communications

Fig. 1

From: An OB-fold complex controls the repair pathways for DNA double-strand breaks

Fig. 1

FAM35A and C20ORF196 form a complex with REV7. a Silver-stained SDS-PAGE gel showing the polypeptides that were immunopurified from extracts of HEK293 cells expressing FLAG-tagged REV7, FAM35A and C20ORF196 using the anti-FLAG antibody. The major polypeptides on the gel (arrows) were identified by mass spectrometry. b–d Immunoblot showing the IP of FLAG-tagged REV7 (b), FAM35A (c) and C20ORF196 (d). IPs were performed with or without 100 μg ml–1 EtBr. The cross-reactive bands were indicated as asterisks. e–g MBP-pulldown (e, f) and GST-pulldown (g) examined the direct interactions among REV7, FAM35A and C20ORF196. The bait and prey proteins were detected with Coomassie blue staining and immunoblotting, respectively. h Schematic representation of FAM35A and POT1

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