Fig. 7
From: Dopamine–endocannabinoid interactions mediate spike-timing-dependent potentiation in the striatum
eCB-tLTP depends on D2R expressed by cortical pyramidal cells. a GFP fluorescence in D2RLoxP/LoxP injected with AAV-cre-GFP in somatosensory cortex. Left panel: site of injection of the AAV-cre-GFP and the resulting fluorescence in pyramidal cells in somatosensory cortex (scale bar: 250 µm). Note that the fluorescence is restricted to neurons within cortex and does not cross corpus callosum (Cc). Right panel: GFP fluorescence in corticostriatal afferents within dorsal striatum (scale bar: 100 µm). b, c D2R immunostaining in the somatosensory cortex of D2RLoxP/LoxP mice injected with AAV-cre-GFP (b) or with AAV-GFP (c); (scale bar: 100 µm). d, e Experiments of plasticity expression in cKO mice for D2R expressed at corticostriatal glutamatergic afferents. d 15 post-pre pairings failed to induce tLTP in D2RLoxP/LoxP mice injected with AAV-cre-GFP in somatosensory cortex (n = 8, 0/8 and 7/8 cells showed tLTP and tLTD, respectively). e As a control, tLTP was induced with 15 post-pre pairings in D2RLoxP/LoxP mice injected with AAV-GFP (n = 5, 5/5 cells showed tLTP). Representative traces are the average of 15 EPSCs during baseline (black traces) and 45 min after STDP protocol (grey traces). Vertical grey dashed line indicates the STDP protocol. Error bars represent sem. **p < 0.01; ***p < 0.001 by one sample t-test (d, e)
