Fig. 2
From: Natural variation at XND1 impacts root hydraulics and trade-off for stress responses in Arabidopsis
A XND1-based association analysis allows refining the natural allelic variations of XND1. a The association with Lpr of 27 polymorphic sites (MAF > 0.05) in the indicated XND1 genomic region was investigated in a set of 112 accessions. The x-axis shows the nucleotide position of each variant, with empty and filled circles indicating INDELs and SNPs, respectively. The y-axis shows the –log10(P) for the association tests, with the significance threshold at α = 0.05 indicated with a dashed line. b The eight polymorphisms selected for further analysis are projected onto a schematic representation of XND1 gene structure. For position −1962, + and − represent an insertion and deletion, respectively. The boxes represent exons, with solid and empty boxes showing translated and untranslated regions, respectively. The SNP at Chr 5-P25,795,349 that surpassed the significance threshold in a is located in the 5’-UTR of XND1 and indicated as SNPUTR. c The eight selected nucleotide polymorphisms (with their distance from translation start site shown on the top) define six haplogroups (H1–H6). Representative accessions and mean Lpr ± SE within each haplogroup (n, accessions number) are shown. One-way ANOVA (Fisher’s LSD, P < 0.05) was used to test the significance of the Lpr data. d Transgenic complementation of xnd1–5 with different allelic forms of XND1. The Lpr of Col-0, xnd1–5, and xnd1–5 plants expressing XND1 genomic fragments from Bur-0, Ty-0, Col-0, or Fei-0, was tested using 3–5 independent transgenic lines per XND1 allele (see Supplementary Figure 5). Mean values ± SE (n = 14–63 plants) are shown with sample size indicated on the top. One-way ANOVA (Fisher’s LSD, P < 0.05) was used to test the significance of the data
