Fig. 4
From: Natural variation at XND1 impacts root hydraulics and trade-off for stress responses in Arabidopsis
XND1-dependent natural variation of Lpr is contributed by effects of SNPUTR on XND1 translation. a–c XND1 transcript abundance was measured in the roots of 49 accessions using 6–10 plants and two independent experiments per accession. Data were analyzed considering each individual accession (a) or their grouping in the H1-H6 (b) or SNPUTR-C vs. SNPUTR-T haplogroups (c). Pearson correlation coefficient (r) and P value (P) between XND1 transcript level and Lpr are shown in a and b. Error bars in b and c indicate SE. Accession number per haplogroup was n = 3–18 in b and as indicated in c (**P < 0.01 in Student’s t test). d A XND1–GFP fusion construct was placed downstream of a CaMV35S promoter and a XND1 5’-UTR, with either C or T at SNPUTR. Note that this SNP is the only variation between Bur-0 and Col-0 XND1 alleles present in the construct sequence. Transcript abundance and fluorescence intensity of XND1–GFP was quantified in the roots of 9–10 transgenic lines per genotype, with two biological replicates. Relative translation efficiency of XND1–GFP was calculated in each individual line from the ratio of fluorescence to mRNA abundance. Mean values ± SE based on total number of lines and repeats are indicated on the right and were normalized to the data for SNPUTR-C. Student’s t test (*P < 0.05) was used to assess the statistical significance of the data
