Fig. 3
From: Using both qualitative and quantitative data in parameter identification for systems biology models
Example constraints used in parameterizing the yeast cell cycle model. The constraints are based on known properties of particular cell phenotypes: Row (i) In a viable cell, the cell must divide before the normalized volume V reaches 8. Row (ii) In a viable cell, the assembly of the mitotic spindle SPN must finish (reach 1). Row (iii) In an inviable cell in G1 arrest, origin activation ORI does not finish (fails to reach 1). Each property was converted to an inequality constraint on one of the outputs of the model. Simulations of cells corresponding to the particular phenotype should be consistent with the constraint (left panels). Simulations of cells corresponding to some other phenotype may not be consistent with the constraint (right panels). Red broken lines indicate that the trace must never exceed the indicated value, and black dash-dot lines indicate the trace must exceed the value at some point. In row iii, the constraint is only enforced to the left of the vertical line, which is located at time τ such that V(τ) = 8
