Fig. 4

49f⁺ Subset1 cells generate 49f⁺ Subset2 cells in vitro. a Sorting strategy used to isolate 49f⁺ Subset1 and 49f⁺ Subset2 populations at day 0 (top panel) and their derived populations after 5 days in culture (bottom panel). b Single cells from each of the indicated gates were sorted. Colours indicate the type of colony produced by each cell after 3 weeks of culture. n = 576 cells plated from three independent CBs for 49f⁺ Subset1 derived populations, n = 576 cells plated from three independent CBs for 49f⁺ Subset2 derived populations. c Percentage of colonies derived from single cells from the indicated day 5 populations. n = 3 independent CBs (except for Diff2 where n = 1). Mean ± SEM is shown. The total number of colonies analysed is shown for each population on the top of each bar