Fig. 2

Expression of Ir-Gal4 drivers in the peripheral nervous system of larval Drosophila. Immunofluorescence with anti-GFP (green) and anti-IR25a (magenta), overlaid on bright-field images, on whole-mount tissue of third instar larvae, revealing the expression of Ir-Gal4 lines in different chemosensory organs (as schematised in the cartoon of the larval head in the centre). Ir7g-Gal4 is expressed in both terminal organ (TO) and ventral organ (VO) neurons; the VO neuron is indicated with an arrow. Images for Ir7b, Ir7e, Ir7g, Ir56c, Ir60c and Ir94e drivers are adapted from28. Genotypes are of the form: w;UAS-mCD8:GFP;IrX-Gal4. Scale bars: 20 μm