Fig. 4
IR56d is expressed in two populations of neurons in the labellum. a Immunofluorescence with anti-RFP (magenta), overlaid on bright-field images, on a whole-mount proboscis of a w;UAS-mCD8:RFP;Ir56d-Gal4 animal. The left image corresponds to the maximal projection of the inner face of one labellar palp, and the right image corresponds to the surface of one labellar palp. Scale bar: 25 μm. b Schematic representing the anatomical location of the taste peg neurons (orange) and taste bristle neurons (blue) in the labellum. c Immunofluorescence with anti-GFP (green), anti-IR25a (blue) and anti-RFP (magenta) on a whole-mount proboscis of a w;LexAop-mCD8:GFP-2A-mCD8:GFP/UAS-mCD8:RFP;Ir56d-LexA/Ir76b-Gal4 animal. The images show a close-up of taste peg neurons (arrowheads) to visualise the co-localisation of the three markers. Scale bar: 25 μm. d Immunofluorescence with anti-RFP (magenta) and anti-GFP (green) on a whole-mount proboscis of a w;LexAop-mCD8:GFP-2A-mCD8:GFP/UAS-mCD8:RFP;NompC-LexA/Ir56d-Gal4 animal. The inset in the merged image shows a bright-field view of the imaged tissue (here and in the following panels). Scale bar: 25 μm. e Immunofluorescence with anti-RFP (magenta) and anti-GFP (green) on a whole-mount proboscis of a w;LexAop-mCD8:GFP-2A-mCD8:GFP/E409-Gal4;Ir56d-LexA/UASCD4:tdTomato animal. Scale bar: 25 μm. f Immunofluorescence with anti-RFP (magenta) and anti-GFP (green) on a whole-mount proboscis of a Gr66a-LexA/+; LexAop-rCD2:GFP/UAS-mCD8:RFP;Ir56d-Gal4/(TM6B or TM2) animal. Scale bar: 25 μm. g Immunofluorescence with anti-RFP (magenta) and anti-GFP (green) on a whole-mount proboscis of a w;LexAop-mCD8:GFP-2A-mCD8:GFP/UAS-mCD8:RFP;Gr64f-LexA/Ir56d-Gal4 animal. Scale bar: 25 μm. h Immunofluorescence with anti-RFP (magenta), anti-GFP (green) and nc82 (blue) on a whole-mount brain of a w;LexAop-mCD8:GFP-2A-mCD8:GFP/UAS-mCD8:RFP;Gr64f-LexA/Ir56d-Gal4 animal. Both left and middle panels show the expression of only the Ir56d-Gal4 driver. The left panel shows the maximal projection of the anterior SEZ; the middle panel shows the maximal projection of the most posterior optical slices of the SEZ. The right panel shows the overlay of the Ir56d-Gal4- and Gr64f-LexA-expressing populations. AMS1 anterior maxillary sensory zone 1, PMS4 posterior maxillary sensory zone 4. Scale bar: 50 μm
