Fig. 2
From: Curvotaxis directs cell migration through cell-scale curvature landscapes
Adherent cells position themselves in the concave areas of cell-scale hills-and-valleys landscapes. a The surfaces were segmented into five areas delimited by isoheights contour lines. b Spatial distribution of different cell types assessed 24 h after surface inoculation on S10/100 surfaces. c, d Spatial distribution of MDCK cells within an epithelium (c) or forming small clusters (d). For the epithelium, the edge is compared with the center of the colony. e–g Surface distribution of mMSCs on homothetically scaled surfaces (e, f) and a surface series of decreasing amplitude (g). h A logarithmic relationship links the cell response, expressed in percentage of cells on concave, to the maximal curvature radius of the surface. i Cell response at 24 h when the surface has been flipped 2 h after cell adhesion. j Cell response on surfaces functionalized with decreasing concentrations of fibronectin. Error bars represent the standard error of the mean number of cells observed in three independent experiments. The total number of cells is indicated for each condition. Scale bar: 100 µm
