Fig. 2
A-D-M mutant and WT PanNETs as two distinct gene expression groups. a Unsupervised clustering of PanNETs using top 3000 variant genes across all samples revealed two distinct robust clusters. The two clusters almost perfectly separate A-D-M WT panNETs from A-D-M mutant panNETs. b Principal component analysis using top 3000 variant genes separated the A-D-M mutant from A-D-M WT PanNETs along the first principal component (PC1). A-D-M mutant panNETs were more homogeneous in gene expression than A-D-M WT as shown by smaller variation along PC1. c Heatmap of pair-wise Pearson correlation of panNETs using top 3000 variant genes across all samples revealed a higher correlation among A-D-M mutants as compared to A-D-M WT panNETs. Red color represents higher correlation and blue represents lower correlation. d Heatmap of top variants genes showing liver, complement, and coagulation genes highly expressed in A-D-M mutant panNETs. Star (*) below sample names represent liver metastatic samples (except for A_mk12 which is a lymph node)
