Fig. 3

Transcriptional activity and expression pattern of three zinc-finger genes. a Subcellular localization of ZnF5-, ZnF7-, and ZnF8-GFP fusion proteins in rice protoplasts. A nuclear marker protein, OsMADS15, fused with RFP, was used as the positive control. Scale bars, 10 μm. b Transcriptional activity assay. The GAL4-BD fusion effectors were constructed using the entire coding region or 3′ truncated (EAR motif deletion) coding region of ZnF5, ZnF7, and ZnF8, respectively. Renilla luciferase reported gene was used as the internal control. Horizontal gray bars show the normalized mean (n = 3 replicates) for each construct, with error bars showing standard deviation. c Expression profiles of the putative genes in the fine-mapped region of SPROG1, as assessed using RNA-seq. d RNA in situ hybridization. Expression patterns of ZnF5, ZnF7, and ZnF8 were measured in the tiller bases at 30 days after sowing. The sense probe was hybridized and used as the negative control. Black arrowheads indicate the position of axillary bud and root primordial in the tiller base. Scale bars, 100 μm