Fig. 4
From: Time units for learning involving maintenance of system-wide cFos expression in neuronal assemblies
cFos-dependent extension of time unit for learning upon local inhibition of protein degradation. Preventing protein degradation locally with proteasome inhibitor is sufficient to extend time window of cFos expression throughout the cFos network. Local delivery of MG132 at 3 h to vH (left); pale colors: vehicle controls. vCA3: Two-way ANOVA, Interaction P < 0.0001, PreLC: Two-way ANOVA, Interaction, P < 0.0001, BLA: Two-way ANOVA, Interaction, P < 0.0001; n = 9, 9, 5, 6, 5, 6. Local delivery of MG132 at 3 h to PreLC (right); PreLC: Two-way ANOVA, Interaction, P < 0.0001, vCA3: Two-way ANOVA, Interaction, P < 0.0001, BLA: Two-way ANOVA, Interaction, P < 0.0001, n = 5, 5, 5, 5, 5, 5. b cFos-dependent extension of time unit function. Left: extension of fear memory binding time unit by local delivery of proteasome inhibitor. vH treatment: Two-way ANOVA, Interaction, P < 0.0001, n = 5 each; PreL treatment: Two-way ANOVA, Interaction, P < 0.0001, n = 5 each. Center: cFos dependence of time unit extension: Two-way ANOVA, Treatment, P < 0.0001, n = 5 each. Right: extension of time unit for trial completion [Two-way RM ANOVA, Interaction, P = 0.0023, n = 5 in each group. Extension of time unit for interference [Two-way RM ANOVA, Interaction, P = 0.0003, n = 5 in each group. Error bars: SEM; P < 0.05 (*), 0.01 (**), 0.001 (***), 0.0001 (****). For a more detailed description of the statistical analysis please refer to Supplementary Table 4
