Fig. 6
From: Targeting of NLRP3 inflammasome with gene editing for the amelioration of inflammatory diseases
Mitigation of MSU-induced peritonitis via CLANmCas9/gNLRP3-mediated NLRP3 knockout. a Therapy scheme and FACS analysis of neutrophil recruitment in the peritoneal cavity of MSU-induced peritonitis. n = 5 per group. b–d Total number of neutrophils infiltrated in the peritoneal cavity (b), ELISA of IL-1β production in peritoneal lavage fluid (c), or immunoblot analysis of NLRP3 knockout efficiency in peritoneal macrophages (d) of mice pre-treated with CLANmCas9/gNLRP3 or other formulations and then challenged with MSU. The data are representative of two independent experiments (a, d) or are shown as the means ± SEM of n = 5 (b, c). Two-way ANOVA (b, c) **P < 0.01, ***P < 0.001
