Fig. 1
From: SHQ1 regulation of RNA splicing is required for T-lymphoblastic leukemia cell survival
Specific high SHQ1 expression in T-ALL. a Heatmap of top 97 highly expressed genes in 117 pediatric T-ALL samples in comparison to 7 normal bone marrow cells (BM). Unsupervised hierarchical cluster was analyzed from GSE26713. A total of 77 T-ALL samples are characterized by oncogenic rearrangements, including TAL/LMO (n = 36), TLX (n = 29), MYB translocations (n = 2), and HOXA activating rearrangements (n = 10). Samples without such abnormalities are identified as Unknown (n = 40). Average gene expression from each category of T-ALL was compared to that in normal BM. Common genes highly expressed in T-ALL are clustered and shown in a descending order as to gene expression levels in T-ALL (left; red indicates increased and blue decreased) (see Supplementary Data 1). Distributions of SHQ1 mRNA expression derived from the heatmap are presented on the right. b, c SHQ1 expression was analyzed among 1036 human cancer cell lines in CCLE database (https://portals.broadinstitute.org/ccle) (b), as well as other primary T-ALLs and normal BM (left, 46 T-ALL samples in GSE28497; right, 11 T-ALL samples in GSE7186) in (c). d Expression analysis of SHQ1 among 2096 primary samples of hematological diseases and BM (GSE13159). e SHQ1 expression was analyzed from gene expression profiling of 64 human T-ALL samples and 4 normal CD4+CD8+ thymocyte samples. Above all, sample numbers are shown in parentheses. The distributions of SHQ1 mRNA expression are presented as log2 median-entered intensity and shown in box-and-whisker plots with the median value (line), the interquartile range (box), and up to 1.5× the interquartile range (bars). Unpaired t-test was used for statistic analysis in (c–e). f Immunoblots of SHQ1 in 3 normal thymus and 5 primary T-ALL patient samples. NOTCH1 mutation status in primary T-ALL is also provided. These samples were obtained from Belgium and labeled as B1–B5. g Immunoblots of murine SHQ1 in normal C57BL/6 thymus cells as well as 2 primary murine T-ALL samples KrasG12D/NOTCH1L1601P (L1601P), KrasG12D/NOTCH1L1601PΔP (L1601PΔP), and 2 murine T-ALL cell lines T6E and G4A2. ACTIN serves as a loading control
