Fig. 3

Multiplexed detection of glucose and asparagine from sweat. Schematic representation (above) and current blockades with its all-point current histogram (below) of two consecutive events showing the entry of SBD1 and GBP into a ClyA nanopore, respectively, under −90 mV applied transmembrane potential. The trans solution contained 5 µL of sweat constituting a 100-fold dilution. The solution used for the electrical recordings contained 150 mM NaCl and 15 mM Tris-HCl set to pH 7.5. Current traces were collected applying a Bessel low-pass filter with a 2 kHz cutoff and sampled at 10 kHz at room temperature (25 °C). A post-acquisition Gaussian filter of 200 Hz was applied