Table 3 Statistical analysis of metastatic cancer cell movements upon inhibition of actin regulators

From: Lévy-like movement patterns of metastatic cancer cells revealed in microfabricated systems and implicated in vivo

Protein

MSD (α)

MLE for μ

Akaike weights

inhibited

 

Truncated power law

TP

P

E

Control

1.54 ± 0.01

2.49 ± 0.07

> 0.99

< 0.01

< 0.01

Cofilin1siRNA

1.56 ± 0.05

3.01 ± 0.14

> 0.99

< 0.01

< 0.01

Profilin1siRNA

1.28 ± 0.01

2.62 ± 0.08

> 0.99

< 0.01

< 0.01

Dia1siRNA

1.51 ± 0.09

2.57 ± 0.11

> 0.99

< 0.01

< 0.01

Arp2/3CK666

1.01 ± 0.03

3.27 ± 0.09

> 0.99

< 0.01

< 0.01

Rac1NSC23766

1.51 ± 0.03

2.51 ± 0.08

> 0.99

< 0.01

< 0.01

Myosin II Blebbistatin

1.83 ± 0.09

1.76 ± 0.07

> 0.99

< 0.01

< 0.01

  1. Table summarizes α exponent values and the maximum likelihood estimates, MLEs70, of the truncated power exponents μ for all treatments (± values give the 95% confidence intervals) described in Fig. 5. For the knockdowns, the values shown correspond to 30 nM siRNA concentrations. For the chemical inhibitors data shown corresponds to: 40 μM CK666 (for Arp2/3), 100 μM NSC23755 (Rac1), 10 μM Blebbistatin (Myosin II). The additional results for all drug and siRNA concentrations tested are shown in Supplementary Figures 10–14. Notice that Arp2/3 inhibition (highlighted in bold) reverts motions to diffusive characterized by α ~ 1 and truncated power law distribution with μ > 3. Lower cutoff values were a = 6 for Control, Cofilin1, and Profilin1 siRNAs, and a = 3 for other treatments. Note that in all cases truncated power law (TP) distribution is favored over power law (P) and exponential (E) distributions, and diffusive-vs-Lévy characteristic is determined by the magnitude of the μ exponent. Analyses based on the following numbers of cells and time points: Control (n = 69 cells, m = 17,569 time points); Cofilin1siRNA (n = 25 cells, m = 7,466 time points); Profilin1 siRNA (n = 29 cells, m = 7,250 time points); Dia1 siRNA (n = 7 cells, m = 2,017 time points); Arp2/3 CK666 (n = 36 cells, m = 9,008 time points); Rac1NSC (n = 26 cells, m = 7,407 time points); Myosin IIBlebbistatin (n = 26 cells, m = 5,413 time points). See Supplementary Movies 9–12 and Supplementary Movies 16–18
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