Fig. 5

Cell autonomous reduction in axon branching of NUAK1-deficient neurons. a–f Histochemistry of the ipsilateral or contralateral side of NUAK1+/+, NUAK1F/+, and NUAK1F/F mice at P21 following in utero electroporation with CRE and the fluorescent protein mVenus. Blue: Hoescht, Red: CUX1 immunostaining. Scale bar: 250 μm. g–h Quantification of normalized mVenus fluorescence in Layer 5 of the ipsilateral cortex (g) and along a radial axis in the contralateral cortex (h). Data: Average ± SEM. NWT = 6, NHET = 13, NKO = 6. Analysis: Mann–Whitney test. ns: p > 0.05