Fig. 1 | Nature Communications

Fig. 1

From: ROS-induced R loops trigger a transcription-coupled but BRCA1/2-independent homologous recombination pathway through CSB

Fig. 1

ROS trigger BRCA2/1-independent, RAD52-, and RAD51-dependent TC-HR. a Schematic diagram of the RAD51 damage response to KillerRed (KR)-mediated ROS-induced damage at transcription on (TA-KR) or off (tetR-KR) genomic loci in U2OS TRE cells (scale bar: 2 μm). b γH2AX foci frequency at TA-KR at early (1 h) or late (36 h) time points after light-induced KillerRed activation in siCtrl and siRAD51-treated cells (scale bar: 2 μm). c RAD51 foci frequency at TA-KR in cells treated with control, BRCA1, or BRCA2 siRNAs (scale bar: 2 μm). d RAD51 foci frequency at TA-KR in WT and RAD52 KO cells (scale bar: 2 μm). e RAD51 foci frequency at TA-KR in RAD52 KO cells and RAD52 overexpressed KO cells. f γH2AX foci frequency at TA-KR at early (1 h) and late (36 h) time points after damage in RAD52 KO cells treated with or without siRAD51. g Model of RAD51 regulation under ROS or nuclease-induced damage at transcribed regions. For a–f, n = 3, 50 cells per replicate. Unpaired t-test, error bars represent SEM. *P < 0.05, **P < 0.01, ***P < 0.001

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