Fig. 5 | Nature Communications

Fig. 5

From: ROS-induced R loops trigger a transcription-coupled but BRCA1/2-independent homologous recombination pathway through CSB

Fig. 5

ROS-induced R loops activate TC-HR through CSB. a Colocalization of CSB or GFP-RAD52 with S9.6 foci at TA-KR (scale bar: 2 μm). b Myc-CSB or GFP-RAD52 foci relative intensity at TA-KR in cells overexpressed with HA-RNase H1 WT or D210N mutant (n = 10 cells in one experiment). c γH2AX foci (36 h) intensity at TA-KR in cells transfected with GFP-RNaseH after damage induction (n = 50 cells in one experiment, scale bar: 2 μm). For b and c, error bars represent SEM. d In vitro binding of CSB and RAD52 proteins to DNA:RNA hybrids in an electrophoretic mobility shift assay (EMSA). e DNA:RNA hybrids EMSA assay of purified GST-CSB-CTD (1200–1493) protein. For d and e, n = 3, error bars represent SD. Unpaired t-test, *P < 0.05, **P < 0.01, ***P < 0.001

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