Fig. 4
From: Galanin neurons in the ventrolateral preoptic area promote sleep and heat loss in mice
Chemoactivation of VLPOGAL neurons by CNO. a Schematic representation of viral vector injections. b Outlines of AAV-hM3Dq injection sites in VLPO at two levels (equivalent to AP: +0.26 and AP: +0.14 in Ref. 70; Animal IDs (n = 15 mice) and the magnitude of hypothermia observed after chemoactivation in each mouse is represented in matching colors c cFos expression (black nuclei) in mCherry-expressing neurons (brown) 3 h after i.p. injections of CNO (0.3 mg/kg) in VLPOGAL-mCherry mice (left) and VLPOGAL-hM3Dq mice (right). d Representative trace of in vitro activation of an hM3Dq-expressing VLPOGAL neuron with 1 μM CNO (n = 5 cells from five mice). 3 V, 3rd Ventricle; AC Anterior commissure; OC optic chiasm; MnPO Median preoptic nucleus; MPO Medial preoptic area; PvPO Periventricular preoptic area; VLPO Ventrolateral preoptic area; VLPOc. VLPO core; VLPOed, VLPO extended dorsal; VLPOem, VLPO extended medial. Scale bars in c 200 μm (upper panels) and 50 μm (lower panels) and d 20 mV, 60 s
