Fig. 3

DAF-16/FOXO and HLH-30/TFEB require each other to promote longevity. Lifespan phenotypes caused by loss of daf-16 and/or hlh-30 in various genetic backgrounds. Red circles indicate the largely absent additive effects caused by joint loss of both transcription factors. a, b Animals of indicated genotypes were grown from the L1 stage at 15 °C and then shifted to 25 °C at the L4 stage and their lifespan was monitored. (n ≥ 98; for detailed statistics including log-rank tests see Supplementary Table 2.) c Animals of the indicated genotypes were grown from the L1 stage at 25 °C and their lifespan was monitored. (n ≥ 173; for detailed statistics including log-rank tests see Supplementary Table 2.) d Models illustrating the genetic interaction between daf-16 and hlh-30 for the promotion of normal lifespan and longevity