Fig. 4
DAF-16/FOXO and HLH-30/TFEB co-regulate a large number of genes, in particular genes that influence aging. a C. elegans with the genotypes wild type, daf-2(e1370ts), daf-16(mu86lf); daf-2(e1370ts), and daf-2(e1370ts); hlh-30(tm1978lf) were grown to the L4 stage at 15 °C, then shifted for 12 h to 25 °C, harvested, and their transcriptomes determined by mRNA-seq. The scatter plots on the left show the genes significantly upregulated and the scatter plots on the right the genes significantly downregulated in daf-2(e1370ts) compared to wild type animals. b C. elegans with the genotypes wild type, glp-1(e2141ts), daf-16(mu86lf); glp-1(e2141ts), and glp-1(e2141ts); hlh-30(tm1978lf) were grown from the L1 stage at 25 °C, harvested as young adults, and their transcriptomes determined by mRNA-seq. The scatter plots on the left show the genes significantly upregulated and the scatter plots on the right the genes significantly downregulated in glp-1(e2141ts) compared to wild type animals. c C. elegans with the genotypes wild type, daf-16(mu86lf), and hlh-30(tm1978lf) were grown to young adulthood at 20 °C, then harvested, and their transcriptomes determined by mRNA-seq. The Venn diagrams illustrate the number of genes significantly regulated in the mutants compared to wild type as well as their overlap. d Based on the data from a, these Venn diagrams illustrate the number of genes significantly regulated in the double-mutants compared to daf-2(e1370ts) as well as their overlap. e Based on the data from b, these Venn diagrams illustrate the number of genes significantly regulated in the double-mutants compared to glp-1(e2141ts) as well as their overlap. (Significance of gene expression changes in a–e was determined by Cuffdiff, using an FDR of 0.05. Significance of gene list overlaps in c–e was determined by Fisher’s exact test.) f–h GO-term enrichment analyses, conducted on the co-activated and co-repressed genes shown in c–e. Only GO-terms of significant enrichment are shown (DAVID score ≥ 1, *: here the term aging was amongst the 12 most enriched GO-terms, but its score was only 0.62 and therefore below the significance threshold). Arrows highlight any aging-related GO-terms, in particular stress responses, protein homeostasis, and aging itself
