Fig. 6
The genetic interaction between daf-16 and hlh-30 is context-dependent. a, b Stress survival phenotypes caused by loss of daf-16 and/or hlh-30. Animals of indicated genotpyes were grown at 20 °C until day 1 adulthood, then transferred to either 6 mM tBOOH (oxidative stress) (a) or to 32 °C (heat stress) (b). (n ≥ 116, for detailed statistics including log-rank tests see Supplementary Table 2.) c Dauer formation phenotypes caused by loss of daf-16 and/or hlh-30 in daf-2(e1370ts) animals. Eggs of indicated genotypes were hedged and grown at 22.5 °C for 3 days. Dauer formation was scored based on developmental arrest and morphology. (n = 100, results from biological triplicates, *: p < 0.05, t-test, error bars indicate s.d.) d Models illustrating the genetic interactions between daf-16 and hlh-30 for the promotion of oxidative stress resistance, heat stress resistance, and dauer formation
