Fig. 7
The context-dependent genetic interactions between daf-16 and hlh-30 can be explained by the distinct distribution of the different response genes between genes individually or co-regulated by these transcription factors. a, b Genes differentially expressed upon oxidative stress or heat stress. Wild type animals were grown at 20 °C until young adulthood, then transferred for 12 h to either 6 mM tBOOH (oxidative stress) (a) or to 32 °C (heat stress) (b), harvested, and their transcriptomes determined by mRNA-seq. The volcano plots illustrate the fold expression changes and their significance for each gene of the transcriptome (n = 20,389). c, d C. elegans with the genotypes wild type, daf-16(mu86lf), hlh-30(tm1978lf), and daf-16(mu86lf); hlh-30(tm1978lf) were grown at 20 °C until young adulthood, then transferred for 12 h to either 6 mM tBOOH (oxidative stress) (c) or to 32 °C (heat stress) (d), harvested, and their transcriptomes determined by mRNA-seq. The Venn diagrams illustrate the number of genes significantly activated by DAF-16 and/or HLH-30, based on the comparison of their mutants to wild type animals (for Venn diagrams of the repressed genes see Supplementary Fig. 6). False coloring illustrates enrichment for genes responding to oxidative stress (c) or heat stress (d), as they were determined in panels a and b, respectively. e The same Venn diagrams as in Fig. 4d, but now with false coloring illustrating the enrichment of dauer formation-related genes (n = 276, based on GO-term and phenotypic annotations in wormbase.org). (Significance of gene expression changes in a–e was determined by Cuffdiff, using an FDR of 0.05)
