Fig. 1
Sensory nerves innervate head and neck squamous cell carcinomas. a En face bright field images of HNSCC nerve “twigs” and “bundle” IHC stained for β-III tubulin (n = 30 patient tumors analyzed) (scale bars, 50 µm); brown, immunostain; blue, counterstain; b En face bright field images of HNSCC IHC stained for Tyrosine Hydroxylase (TH), Vasoactive Intestinal Polypeptide (VIP) and Transient Receptor Potential Vanilloid-type 1 channel (TRPV1) (scale bar, 20 µm) (n = 15 additional patient tumors analyzed for these markers). Brown, immunostain; blue, counterstain. Representative images shown. c En face bright field representative IHC images of human HNSCCs for β-III tubulin (pink, arrows) and TRPV1 (brown, arrowheads). Low magnification, scale bar, 50 μm. Higher magnification, scale bar, 20 μm. n = 12 patient tumors were co-stained with representative images presented. d Quantification of β-III tubulin/TRPV1 IHC stained human samples described in c. Dark gray bars, number of TRPV1 and β-III tubulin positive twigs; light gray bars, total number of β-III tubulin positive twigs
