Fig. 4

Sensory innervation of mEERL tumors. a IHC staining of mEERL tumors for β-III tubulin (n = 30); Tyrosine Hydroxylase (TH), Vasoactive Intestinal Polypeptide (VIP), and Transient Receptor Potential Vanilloid-type one (TRPV1) (n = 15 additional tumors analyzed for each marker) (scale bar, 20 µm). Brown, immunostain; blue, counterstain. b IHC stained serial sections of a mEERL parental tumor for β-III tubulin (neuronal marker) and cytokeratin (epithelial marker), scale bar, 50 µm. c Triple immunofluorescent labeling of mEERL tumor for Tau (green), β-III tubulin (red), and TRPV1 (far red, seen as white); merged image presented (merge). Nuclei counterstained with DaPi (blue). Scale bar, 10 µm