Fig. 7

HPV status and EphrinB1 contribute to neurite outgrowth. a Quantified neurite outgrowth of PC12 cells following exosome stimulation with HPV negative (−) or positive (+) human cell lines; statistical analysis by one-way ANOVA with post hoc Tukey test for differences between HPV- and + groups; *p<0.001. The variance between groups compared is similar. n = 3 technical replicates/condition; experiment repeated at least two times. Central value was the mean. Comparisons between individual groups were analyzed by one-way ANOVA with post hoc Fisher’s Least Significant Difference (LSD) test. All comparisons and LSD p values found in Supplementary Table 5. b Quantified neurite outgrowth of PC12 cells following exosome stimulation from HPV(−) SCC1 parental cells or SCC1(−) EphrinB1 over-expressing cells [SCC1(−) EphrinB1]. Statistical analysis by one-way ANOVA with post hoc Fisher’s Least Significant Difference (LSD) test; LSD p values reported, *p<0.02. The variance between groups compared is similar. n = 4 technical replicates/condition; experiment repeated twice. Central value used was the mean. All comparison and LSD p values found in Supplementary Table 6. c Western blot analysis of whole tumor lysates from mice bearing SCC1 or SCC1 EphrinB1 tumors. Western blots repeated at least n = 4 times with technical replicates. Western blots have been cropped for clarity and conciseness. Densitometric quantification of western blot in panel c for d β-III tubulin, e Tau, and f TRPV1. Statistical analysis by unpaired, two-tailed Student’s t-test; *p<0.03; ns not significant. The variance between groups compared is similar. Central value was the mean. g Quantified neurite outgrowth of PC12 cells following stimulation with exosomes from HTE, human tonsil epithelia; HTE E6ΔE7, cells expressing HPV16 E6 deleted of its PDZBM(Δ) and E7; HTE E6E7, cells expressing HPV16 E6 and E7. Statistical analysis by one-way ANOVA with post hoc Fisher’s Least Significant Difference (LSD) test, LSD p values reported, *p<0.0001; ns, not significant. The variance between groups compared is similar. Central value was the mean. n = 3 replicates/condition; experiment repeated twice. All comparisons and LSD p values found in Supplementary Table 6. All error bars are standard deviation