Fig. 2
BDNF synthesis and intracellular transport were impaired in various HD models. a Schematic diagram showing that BDNF is modulated at synthesis, transport, and secretion levels. b Hippocampal BDNF protein level determined by ELISA in R6/1 and HdhQ111/Q111 mice; values are mean ± s.e.m (% of WT); n = 21 and 14 mice for WT and R6/1; n = 6 and 9 mice for WT and HdhQ111/Q111, respectively. c Representative kymographs of intracellular transport of BDNF-containing vesicles (white trajectories) in a neurite (50 μm from soma) over 75 s in wHTT- and polyQ-HTT-expressing rat hippocampal neurons. The velocity of BDNF transport was reflected by the slope of trajectories (moving distance against time). d–f Anterograde and retrograde BDNF transport velocity in all neurites of wHTT- and polyQ-HTT-expressing rat hippocampal neurons (d) and hippocampal neurons from R6/1 mouse line (e) and in the axon of hippocampal neurons from HdhQ111/Q111 mouse line (f); values are median ± 95% c.i. (d, e) or mean ± s.e.m (f); n = 5569, 5656, 5227, and 5706 trajectories for anterograde and retrograde wHTT and polyQ-HTT, respectively; n = 1424, 1710, 1376, and 1487 trajectories for anterograde and retrograde WT and R6/1, respectively; n = 236, 261, 194, and 256 trajectories for anterograde and retrograde WT and HdhQ111/Q111, respectively. Significance was determined by unpaired two-tailed Student’s t test (b, f) or two-tailed Mann–Whitney test (d, e); *P < 0.05, **P < 0.01, ***P < 0.001
