Fig. 6
AMPAR fails to stabilize on the neuronal surface after LTP stimulation, an effect rescued by tianeptine (TIA) treatment in an HD cellular model. a, c, e Typical GluA2 trajectories (magenta) before and after cLTP induction in FL-wHTT-expressing neurons without TIA treatment (a), FL-polyQ-HTT-expressing neurons without TIA treatment (c), and FL-polyQ-HTT-expressing neurons with TIA treatment (10 μM) (e). Scale bar, 5 μm. b, d, f The ratio of mobile to immobile fraction of the diffusion coefficient (D) at synapses before and after cLTP induction in FL-wHTT-expressing neurons without TIA treatment (b), FL-polyQ-HTT-expressing neurons without TIA treatment (d), and FL-polyQ-HTT-expressing neurons with TIA treatment (10 μM) (f). Data were from 10 to 14 neurons from three separate experiments. Immobile fraction was identified as the proportion of receptors with D ≤ 0.01 μm2 s−1 while mobile fraction with D > 0.01 μm2 s−1. Paired t test was used; *P < 0.05; **P < 0.0;1; ns not significant
