Fig. 3
From: TRPV4 and KRAS and FGFR1 gain-of-function mutations drive giant cell lesions of the jaw

MAPK pathway activation in GCLJ. Immunohistochemical (IHC) staining for phospho-ERK1/2 shows strong positive staining in mononucleated cells in GCLJ lesions. Representative images of phospho-ERK1/2 staining in TRPV4 p.M713V (a), KRAS p.G12D (b), and FGFR1 p.C831R (c) mutant and in WT (d) GCLJ cases. e Immunoblot showing sustained phospho-ERK1/2 (pERK1/2) activation in TRPV4 M713I HEK293 mutant cells compared to WT. A representative experiment of three independent assays is provided. Scale bar represents 200 μm