Fig. 3

Analysis of intercellular transport rates as a function of cell-cycle state. a Asynchronous cell population (Async) shifted to G2-phase after nocodazole treatment (NOC), and after 45 min of recovery (NOC45) the population shifted to G1-phase. b The population distribution averages from a were calculated for the Async, NOC, and NOC45 treatment conditions. c An illustration of the GAP-FRAP technique for quantification of relative diffusion rates between adjacent cells. d A histogram of recovery time constants collected using gap-FRAP in the Async population (n = 64), where high and low recovery constants represent slow and fast transport rates, respectively. e The distributions of recovery constants for Hoechst-identified mitotic (HMI) cells (n = 26) and NOC45 (n = 28) were shifted to the right and left of the Async population, respectively, indicating slower and faster transport in these populations. f A projection of each cell cycle state onto the Async distribution using information from a and e, as described in Supplementary Figure 3