Fig. 4

Computational analysis of cell-cycle modulation on intercellular communication. a Each cell type (P, pluripotent; d, differentiated) has a base permeability, representing the average percent of gap junction hemichannels open at a cell-cell interface, with the total percent of open channels being the product of the two base permeabilities. b A function for cell-cycle modulation over time was defined based on the transport trends noted in Fig. 3, with a convolution of all possible transport profiles that two cells could experience over time for P-P, P-D, and D-D. c The intercellular transport model was implemented within an agent-based model and compared to digitized experimental colonies over time. Supplementary Movie 1 is a representative video of the progression of intracellular gradient formation and differentiation