Fig. 4

Host-associated C. rodentium metabolism co-ordinated with infection. a Networks graphs illustrating the identification of gene ontology (GO) terms in vivo relating to Supplementary Data 4. Networks were generated for both upregulated and downregulated genes individually. Coloured nodes represent GO terms associated with the DEGs in vivo and the arrows indicate relatedness to one another by distance. Significantly enriched GO terms (P ≤ 0.05) were assigned a numbered node and each node is colour-coded to represent enrichment of a biological function at the caecum (red), the rectum (blue) or at both sites (black). The identification of 1,2-propanediol as a significantly enriched biological process in vivo is indicated at node 26. The colour coding of related nodes is indicated on the inset legend. b The top 10 DEGs identified at peak infection according to fold increase in expression (EDGE test). Genes from the pdu operon (1,2-propanediol metabolism) are highlighted in blue and the relative EDGE test P-value indicated above. Each bar represents the mean of 3 biological replicates. c Growth of C. rodentium and ∆pduA-J with 1,2-propanediol (1,2-PD) or propionate (Prop) as a sole carbon source. Data points indicate the mean optical density measurements (600 nm) of three biological replicates ± SEM. d The C. rodentium pdu operon. Genes are colour-coded according to role in 1,2-propanediol metabolism. The figure is adapted from Sturms et al.^80. e Schematic depiction of the 1,2-propanediol metabolic pathway. The intermediate products and genes involved in their generation are indicated