Fig. 6

In silico modeling of P4- and P5-associated ACTB variants. a Overview of wildtype (WT) β-CYA (mid) in cartoon with space-fill overlay. β-CYA^P4 and β-CYA^P5 to the left and right respectively, with missing volumes indicated in yellow space-fill and affected residues modeled in magenta space-fill; b The binding interface of NM-2 (blue, according to PDB 5JLH) on a β-CYA^WT F-actin triplet (mid, green with magenta C-terminal residues) is shown. Close-ups of affected β-CYA^P4 (left) and β-CYA^P5 (right) residues are superimposed as magenta cartoon structures on the green β-CYA^WT cartoon structure. CM-loop interactions with mutant actins are shown in the top panels, whilst supporting-loop interactions are modeled in the lower panels; c In silico modeling of the interaction interface of β-CYA^WT (green) with α-actinin (orange, according to PDB 3LUE, confirmed by docking) is shown (mid). Left and right show the interface with affected C-terminal residues for β-CYA^P4 (left) and β-CYA^P5 (right), respectively