Fig. 3

CG pathway is the primary pathway for fast endocytic response. a Fluid uptake in wild-type (WT MEF), caveolin-null (Cav^−/−) or conditional Dynamin triple knockout MEFs (Dyn TKO) for 90 s using TMR-Dex at steady state (control) and immediately after relaxing the stretch (stretch–relax). Images (left) show representative cells used to generate the box plots (right) which provide a quantitative measure of the extent of endocytosis of TMR-Dex for the indicated treatments. The uptake on stretch–relax is normalized to the steady-state uptake in the respective cell lines (n = WT MEF–Control (117), Stretch–Relax (123); Cav^−/−–Control (173), Stretch–Relax (187); Dyn TKO–Control (179), Stretch–Relax (177)). b Fluid uptake in CHO cells treated with DMSO (Control) or with LG186 (10 µg/ml) (to inhibit GBF1) for 30 min, either at steady state (steady state) or immediately after relaxing the stretch (stretch–relax). Images (left) show representative cells used to generate the box plot (right) which provide a quantitative measure of the extent of endocytosis of TMR-Dex for the indicated treatments, normalized to the control steady-state condition (n = Control–Steady state (170), Stretch–Relax (189); LG186–Steady state (248), Stretch–Relax (210)). Box plot shows median, 25th and 75th percentile, and whiskers show the standard deviation. Individual data points are overlaid on box plot where each data point is the mean intensity per cell. The ‘n’ indicates total number of cells in each condition pooled from two different experiments with duplicates per experiment; *p < 0.001, ns not significant by Mann–Whitney U test. Scale bar, 10 µm