Fig. 3
Icilin and DMPP synergistically lower body weight in DIO mice via central mechanisms. a Effects on body weight, b cumulative food intake, and c cFOS positive neurons in the hypothalamus following daily s.c. injections to DIO male C57Bl6j mice of vehicle (black, n = 8 for a, b; n = 6 for c), DMPP 10 mg/kg (red, n = 8 for a, b; n = 6 for c), icilin 5 mg/kg (blue, n = 8 for a, b; n = 6 for c), or the combination of DMPP 10 mg/kg and icilin 5 mg/kg (purple, n = 7 for a, b; n = 8 for c) for 4 days (a, b) or 3 days (c), respectively. d cFOS positive neurons in the hypothalamus following daily s.c. injections to DIO male WT C57Bl6j mice or to DIO male CHRNB4 KO mice of vehicle (black, n = 5/gray, n = 4) or the combination of DMPP 10 mg/kg and icilin 5 mg/kg (purple, n = 5/light purple, n = 5) for 3 days. e Effects on body weight and f cumulative food intake following daily s.c. injections to HFD-fed male MC4R KO mice of vehicle (black, n = 8), DMPP 5 mg/kg (red, n = 8), icilin 5 mg/kg (blue, n = 8), or the combination of DMPP 5 mg/kg and icilin 5 mg/kg (purple, n = 7) for 7 days. All scale bars are 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA (a, e) and one-way ANOVA with Tukey post-hoc test (b, c, d, f). All data are presented as mean ± SEM
