Fig. 4
Icilin and DMPP combination therapy increases sympathetic nervous system-linked BAT thermogenesis. a Effects on body weight and b cumulative food intake following daily s.c. injections to DIO male betaless mice or DIO male WT C57Bl6j mice of vehicle (black, n = 8/gray, n = 8) or the combination of DMPP 5 mg/kg and icilin 5 mg/kg (purple, n = 8/bordeaux, n = 7) for 7 days. c Effects on body weight and d cumulative food intake following daily s.c. injections to global UCP1 KO DIO male mice or DIO male C57Bl6j control mice of vehicle (black, n = 7/gray, n = 7) or the combination of DMPP 5 mg/kg and icilin 5 mg/kg (purple, n = 7/light blue, n = 7) for 7 days. e Mean optoacoustic signal intensity and f blood oxygen saturation (SO2) measured by Multi-Spectral Optoacoustic Tomography (MSOT) in selected BAT regions of male C57Bl6j control mice of vehicle (black, n = 6), DMPP 10 mg/kg (red, n = 6), icilin 5 mg/kg (blue, n = 6), or the combination of DMPP 10 mg/kg and icilin 5 mg/kg (purple, n = 6). (g–a to g–d) MSOT image of BAT at 800 nm (gray scale) with overlaid of SO2 (green-red scale) in: a control- (g–a), DMPP- (g–b), icilin- (g–c), and combination-injected mouse (g-d). All scale bars are 5 mm.*p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA (a, c), one-way ANOVA (e, f) with Tukey post-hoc test and two-tailed Student’s t-test (within genotype) (b, d). All data are presented as mean ± SEM
