Fig. 5

Icilin and DMPP reverse obesity and increase BAT UCP1 at thermoneutral conditions. a Effects on body weight, b cumulative food intake, changes in c fat mass and d lean mass, e BAT H & E staining, and f UCP1 immunoreactivity staining in BAT with g quantification of UCP1 immunoreactivity staining (% area). BAT expression of genes involved in h thermogenesis, i mitochondrial transport, and j creatine signaling pathway following daily s.c. injections of vehicle (black or white, n = 8), DMPP 5 mg/kg (red, n = 7), icilin 5 mg/kg (blue, n = 8), or the combination of DMPP 5 mg/kg and icilin 5 mg/kg (purple, n = 7) to DIO male C57Bl6j chronically housed at 30 °C for 14 days. All scale bars are 50 µm. *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA (a) and one-way ANOVA (b, c, d, g, h, i, j) with Tukey post-hoc test. All data are presented as mean ± SEM