Fig. 8

Induction of pG1090::icals3m increases callose which decorates cellulose microfibrils. DMSO-treated and estradiol-treated icals3m roots were co-stained with aniline blue (cyan) and direct red 23 (red) which fluorescently stain callose and cellulose, respectively. a, b show confocal images of a region in the elongation zone of the root 24 h after treatment with DMSO (control). b and close-up show the merged signal at higher magnification. c–f show comparative regions in icals3m roots 24 h after estradiol induction (activation of icals3m). Notice the increased accumulation of callose (cyan signal) in c comparing to a. Consult also Supplementary Fig. 9. d, e and f show enlargements of a cell from c, with their associated close-ups in the separate channels (d, direct red 23); e, aniline blue and f, merged image). Arrowhead highlights an area where callose integrates very closely to cellulose microfibrils. Scale bars (a, b) = 25 µm; (b, d, e, f) = 10 µm; (b, d, e, f close-ups) = 5 µm