Fig. 3
From: Imaging neural activity in the ventral nerve cord of behaving adult Drosophila
Neural activity of dMANs in the thoracic cervical connective during behavior. a Confocal image of MAN-Gal4 driver line expression in the brain and VNC. Scale bar is 40 µm. Neuronal GFP (yellow) and neuropil (nc82, blue) are labeled. A dashed white line highlights the thoracic x–z plane imaged. b Coronal section of the thoracic cervical connective in an animal expressing GCaMP6s (cyan) and tdTomato (red) in MANs (MAN > GCaMP6s; tdTomato). Scale bar is 3.5 µm. c Separated ROIs (top-left) and associated fluorescence signals from right and left dMANs (top-right). Corresponding forward, sideways, and rotational velocities of the fly (bottom-right). Events are indicated as dashed gray lines. d Summary of dMAN activity and spherical treadmill rotations with respect to fluorescence events (748 left neuron and 746 right neuron events) aligned to 0 s (dashed gray line). Control data in which events are time-shuffled are overlaid in gray. Shown are the means (solid line) and bootstrapped 95% confidence intervals (transparencies)
