Fig. 2
Overexpression of the BRL3 receptor promotes root hydrotropism. a Root curvature (hydrotropic response) in 7-day-old roots after 24 h of sorbitol-induced osmotic stress (270 mM). Scale bar: 0.2 cm. b Discrete distribution of root hydrotropic curvature angles in the different genotypes. Lightest green depicts roots curved between 0° and 10°, light green between 10° and 20°, dark gray between 20° and 30°, and darkest green depicts roots that have a curvature of more than 30° . c Continuous distribution of root curvature angles. Different letters indicate a significant difference (p-value < 0.05) in a one-way ANOVA test plus Tukey’s HSD test. Boxplot represent the median and interquartile range (IQR). Whiskers depict Q1 – 1.5*IQR and Q3 + 1.5*IQR and points experimental observations. Data from four independent biological replicates (n > 50). d Stress traits matrix for all physiological assays performed on the roots and shoots of WT, BR loss-of-function mutants and BRL3ox. Root growth in control conditions is highlighted in green. Color bar depicts values for scaled data
