Fig. 5
From: A RNA producing DNA hydrogel as a platform for a high performance RNA interference system
Gene-silencing effect by I-gel i n a cellular level. a Fluorescence images of MDCK-GFP-expressing (MDCK-GFP) cells coincubated with I-plasmid, I-plasmid complexed with lipofectamine, and I-gel. Scale bar: 20 μm. b Fluorescence-activated cell sorter analysis of GFP-expressing cell line, MDCK-GFP cells after treatment of sample/polymerase complexes in serum-deficient medium. The number means the percentage of GFP-overexpressing cells sorted within a prefixed gate region as indicated by a bar. c, d The relative amounts of (c) GFP mRNA and (d) shRNA from GFP-expressing MDCK cells after incubation in various conditions. (†concentration of these conditions were 102-fold increased in consideration of the template to RNA transcription rate of the I-gel) (c: *P < 0.05, analyzed by one-away ANOVA, followed by Holm–Bonferroni multiple comparisons post test. Asterisks indicate statistically significant differences between between each condition and Cell-only) (d: *P < 0.05 and **P < 0.005, analyzed by t test. Asterisks indicate statistically significant differences between each condition and I-gel). Error bars refer to standard deviations from three (c) or six (d) replicates
