Fig. 2
From: Diffusion-limited association of disordered protein by non-native electrostatic interactions
Ionic strength dependence of binding kinetics. a Donor and acceptor fluorescence trajectories (left), FRET efficiency trajectories (middle), and FRET efficiency histograms (right) of TAD/NCBD binding at various NaCl concentrations. Bin time is 1 ms and quoted numbers in the FRET efficiency histograms are NaCl (left) and NCBD (right) concentrations. Red arrows indicate photobleaching of the acceptor. b Donor and acceptor fluorescence trajectories (left), FRET efficiency trajectory (middle), and FRET efficiency histogram (right) of binding of barnase and barstar (20 nM) at 0 mM NaCl. Bin time is 20 ms. c, d Association (red) and dissociation (blue) rate coefficients of c TAD/NCBD and d barnase/barstar binding
